A generalized kinetic model for amine modification of proteins with application to phage display
نویسندگان
چکیده
منابع مشابه
A generalized kinetic model for amine modification of proteins with application to phage display.
Amine modification of filamentous virions (phage particles) is widely used in phage display technology to couple small groups such as biotin or fluorescent dyes to the major coat protein pVIII. We have developed a generalized kinetic model for protein amine modification and applied it to the modification of pVIII with biotin and the near-infrared fluorophor Alexa Fluor 680. Empirically optimize...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2009
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113074